| Sydney Mikayla | Robby Malto / Crowd / Drummer | |
| Kathreen Khavari | Twitch / Crowd / Agent Bagheri / Female Driver / GHOST Agent #2 / Students / Townspeople | |
| Danny Pudi | Bumblebee / Crowd / Dramatic Voice #2 / Smart Trainer 5007 / Smart Trainer 5008 | |
| Cyrus Arnold | Jawbreaker | |
| Stephanie Lemelin | Hashtag / Protoform Hashtag | |
| Zachary Infante | Nightshade / Crowd / GHOST Agents | |
| Alan Tudyk | Optimus Prime / Photographer / Plain Clothes GHOST Agent / Crowd | |
| Rory McCann | Megatron | |
| Cissy Jones | Elita-1 / Computerized Voice / V.A.L. / Crowd / Elita -1 / Dramatic Voice #1 | |
| Nolan North | Swindle / Hardtop / Blitzwave / Crowd / CB Radio / Farmer / Funnel Cake Employee / GHOST Agent / GHOST Agent #3 / PA Announcement / Ride Attendant | |
| Steve Blum | Starscream / Razor-Fin / Soldiers | |
| Diedrich Bader | Mandroid / Crowd | |
| Kari Wahlgren | Agent Croft / GHOST Agent / PA System | |
| Eric Bauza | Additional Voices / Prowl / Kid / Shadowy Figure | |
| Marc Evan Jackson | Agent Schloder / Delivery Man | |
| Flea | Aftermath | |
| Nicole Dubuc | Nova Storm / Skywarp, executive producer, developed by / developed for television by / story editor / story by / written by | |
| Troy Baker | Shockwave | |
| Clancy Brown | Quintus Prime | |
| Siena Agudong | Izzy / Quintesson Shapeshifter | |
| Jake Green | Laserbeak / Crowd / Mugger / Mole Bot / Mole Bots / Townspeople | |
| Zelda Williams | Spitfire | |
| Roger Craig Smith | Breakdown | |
| Kaitlyn Robrock | Cadet Rosato / Crowd / Taylor | |
| Claudia Black | Terratronus | |
| 'Weird Al' Yankovic | Cosmos | |
| Fred Tatasciore | Quintesson Executioners / Quintesson Executioner | |
| Jason Marsden | Cadet Conway / GHOST Agent #1 / Holographic Child | |
| Richard Ayoade | Fairmaestro / Ticket Booth Operator | |
| Krizia Bajos | Agent Bala |
