Her first adult film was Wild at Heart from 1991 (not to be confused with the 1990 David Lynch Wild at Heart film by the same name) with only women. In 1993, Chasey did her first hardcore movie, The Original Wicked Woman, for Wicked Pictures, famous as the first adult video to document a breast enhancement, before and after.
In 1994, she became a contract girl for Vivid Entertainment, where she gained her major success with titles like Chasey Loves Rocco and Chasey Saves the World. She had her own line of lesbian videos, the Chasin' Pink series.
Some mainstream appearances were in 1995 Tales from the Crypt's Demon Knight, 1997 Trey Parker's Orgazmo, and 1998 Spike Lee's He Got Game.
In 2000, The Bloodhound Gang released a song titled "The Ballad of Chasey Lain" a song regarding a series of sexually explicit letters the singer has written to Lain.
In 2002, she did her last film with Vivid Entertainment, Chasin' Pink 6, and took a break from the industry. She came back two years later with 2004 Chasey's Back. Chasey went on to perform in her first interracial scene with Mr. Marcus in Black in White 2 in 2005.
Lain has a son named Thomas, born in 1998.
She is married to Anthony Case.
Returned to the adult film industry in the spring of 2001 after taking three years off to have and raise a son, whom she named Thomas.
The band Bloodhound Gang made a song called "Ballad Of Chasey Lain", a tribute to her. She was supposed to be in the video for the song, but never showed up.