2000 establishments in the Netherlands

Guerrilla B.V. (trade name: Guerrilla Games) is a Dutch first-party video game developer based in Amsterdam and part of PlayStation Studios. The company was founded as Lost Boys Games in January 2000 through the merger of three smaller development studios as a subsidiary of multimedia conglomerate company Lost Boys. Lost Boys Games became independent the following year and was acquired by Media Republic in 2003, renaming the studio to Guerrilla Games before being purchased by Sony Computer Entertainment in 2005. As of June 2021, the company employs 360 people under the leadership of joint studio directors Jan-Bart van Beek, Joel Eschler and Hella Schmidt. It is best known for the Killzone and Horizon game series.

Untamed is a Rocky Mountain Construction hybrid steel-wood roller coaster at Walibi Holland, a theme park in the Netherlands. Untamed replaced Robin Hood, a wooden roller coaster that closed on 28 October 2018.

Team Liquid is a multi-regional professional esports organization based in the Netherlands that was founded in 2000. They signed their first professional players with the release of StarCraft II: Wings of Liberty.

SkyTeam is one of the world's three major airline alliances. Founded in June 2000, SkyTeam was the last of the three alliances to be formed, after Star Alliance and Oneworld. Its annual passenger count is 624 million customers (2024), the second largest of the three major alliances. As of February 2025, SkyTeam consists of 18 active carriers; it also operates a cargo alliance named SkyTeam Cargo, which partners with ten carriers, all SkyTeam members. Its centralised management team, SkyTeam Central, is based in Amstelveen, Netherlands.

The Drents-Friese Wold National Park is a national park in the Dutch provinces of Friesland and Drenthe, covering more than 61 km (24 sq mi), founded in 2000. It consists of forests, heath lands and drift-sands.

ASPIDER is the group name for a series of companies that are mostly based in Europe. The company name has evolved over the years as a result of acquisitions, mergers and restructuring. The company is an MVNE (Mobile Virtual Network Enabler), providing mobile services to companies that want to control their own network. Clients include enterprises, manufacturers, integrators, and the mobile operators themselves.

Zoomin is a media and entertainment company located in Schiphol-Rijk, the Netherlands. Zoomin specializes in custom video production for commercial use.

Suitsupply is a Dutch men's suit and fashion brand founded in 2000 by Fokke de Jong in Amsterdam.

The Via Vinci University, short for “the International University for Creative Leadership and Entrepreneurship”, was intended as an international, Netherlands based private university. The name was used in 2000, it now operates under the name of Via Vinci Academy after scrutiny from the Dutch government. It starting using the name in 2007 when the parent institutes IMAGO Group and the International University of Entrepreneurship merged. The name “Via Vinci” refers to "the way" of Leonardo da Vinci, the icon of universal, innovative and creative strength.

NPO Zappelin is a Dutch television program block for younger children that launched as Z@ppelin in September 2000.