Cameo: (Billy Dee Williams) Judge Reinhold, played Lando Calrissian.
When dedicating the statue of Captain Kirk versus Kahn, Linus jokes that it looks nothing like either of them, to which Seth Rogen's character states "Yes, thank you for pointing that out. Unfortunately the whores at Viacom threatened to sue if we used their likenesses..." This is an in-joke, due to the fact that none of the "Trekkies" wear official Star Trek clothing and the Star Fleet symbol looks nothing like the one from Star trek.
The passenger side door of the van has the word "fanboys" written on it in the 'Star Wars' Aurebesh alphabet and mirrored.
Jay Baruchel's hairstyle and eyeglasses are meant to evoke George Lucas' appearance in the 1970s.
The "Fanboys" theatrical release poster (the unidentified male in a knit shirt looking toward his right shoulder in a Darth Vader helmet, against an orange sunburst background) is a spoof of the theatrical release poster for The 40 Year Old Virgin (2005) (with 'Steve Carrell' appearing in similar clothing and posing against a similar background), the idea being conveyed that fan boys are the quintessential "40-year-old virgins".
The sound effect made by the van during "hyperjump" is the same as R2D2's fast sound effect.
The town they live in is Shandal, Ohio, which is an anagram of Ashland, Ohio.
Jason Mewes: emerges from the gas station bathroom and yells at Kevin Smith.
During the fight between our heroes and the Star Trek (1966) fans at the dedication of the Captain James T. Kirk statue, Spock's ears are ripped off. This is reminiscent of the 1976 Saturday Night Live (1975) skit titled "The Last Voyage of the Starship Enterprise" where Spock's (played by Chevy Chase) ears are removed as Spock calls out, "My ears!", as part of the dismantling of the Star Trek (1966) set and series cancellation.
Billy Dee Williams: Judge Reinhold, played Lando Calrissian.
The Canadian rock band Rush is featured somewhat prominently in several scenes in the movie. Rush is a group that has developed a very large, very cult-like following of mostly male "fanboys".
According to UrbanDictionary.com, the "Mississippi Handbag" is an in-joke inserted by the film's writers.
Carrie Fisher: Doctor, played Princess Leia.
The additional shots made in November and December 2007, weren't done by original director Kyle Newman but by Steven Brill.
The coffee shop where Windows meets Rogue Leader is called "Java the Hut." This isn't just a clever play on words involving the Star Wars character, it's also the name of the coffee shop where Kristen Bell's character worked in Veronica Mars (2004).