John Edwards Holbrook (December 31, 1796 – September 8, 1871) was an American zoologist, herpetologist, physician, and naturalist, born in Beaufort, South Carolina, the son of Silas Holbrook, a teacher, and Mary Edwards. Although Holbrook's memoir, written by his medical partner, and his tombstone both give the date 1794 for his birth, this is incorrect. Holbrook received his A.B. degree from Brown University in 1815, and his M.D. from the University of Pennsylvania in 1818. In 1827, he married Harriott Pinckney Rutledge (1802–1863), granddaughter of John Rutledge and a member of the Middleton-Rutledge-Pinckney family.